The 11 reference contexts in paper N. Shadrova B., O. Pruntova V., G. Skitovich S. (2018) “Proteomic properties of Salmonella isolates” / spz:neicon:veterinary:y:2016:i:2:p:35-38

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    Enteritidis «Глеб» цыплята Salmonella Enteritidis мясо пельменное «Pel» 7,097TyphimuriumSalmonella Typhimurium «комбикорм No 16» lar protein fraction (direct protein profiling), i.e. without fractionation and protein purification, and obtain highly accurate and high resolution mass-spectra, unique for this species, characterizing the tested object on the “fingerprint pattern” principle
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    . Spectra comprising a mass-range of 2–20 kDa are usually used for microbial identification. Analysis of massspectra of E. coli in this range demonstrated only 30 out of 2000 proteins, calculated basing on E. coli sequencing data, in spectra [11].
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    Spectra comprising a mass-range of 2–20 kDa are usually used for microbial identification. Analysis of massspectra of E. coli in this range demonstrated only 30 out of 2000 proteins, calculated basing on E. coli sequencing data, in spectra
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    [11]
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    . Most of the obtained peaks were referred to ribosomal proteins, and the rest to DNA-binding proteins and cold shock proteins. Ribosomal proteins are quite conservative and that makes them taxonomically specific.
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    Besides, many ribosomal proteins are located in cell cytoplasm – up to the half mass of a growing cell, and their set remains unchanged not depending on external conditions and growth stage which ensures mass-spectrum reproducibility. Studies of intra- and inter-laboratory reproducibility demonstrated high reliability of MALDITOF method
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    . The study was aimed at proteomic properties of Salmonella isolates recovered from food products and feeds. MATERIALS AND METHODS Isolates. 27 Salmonella isolates recovered from foodstuffs and feeds in the Russian Federation by the FGBI «ARRIAH» microbiology laboratory in 2006–2010.
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    The main source of human Salmonella infection is animal and plant products (meat, eggs, dairy products, fruit and vegetables). The modern area of foodstuff production, the so called “organic” farming, also increases risk of food poisoning including salmonellosis
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    . Within the last decade microbial identification of protein profiles or direct protein profiling has been used more frequently together with classical, and molecular and biological methods of microbial identification.
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    Within the last decade microbial identification of protein profiles or direct protein profiling has been used more frequently together with classical, and molecular and biological methods of microbial identification. This method is competitive in such criteria as identification accuracy and specificity but it is more rapid and cost effective
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    [2, 3, 9, 10]
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    . Time-of-light mass spectrometry MALDI (MALDI-TOF) is based on matrix assisted laser desorption and ionization of the tested substance followed by ion separation using time-of-light mass-analyzer.
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    When exposed to laser the matrix crystallized with the tested material actively absorbs laser irradiation which leads to its desorption. When transiting to gas-phase the matrix carries molecules of the tested substance and facilitates their ionization with formation of singly charged ions
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    . The method enables to perform direct mass-spectrometry of microbial celluSUMMARY The paper presents results of Salmonella of studying Salmonella isolate properties by MALDI-TOF method using MALDI Autoflex III mass-spectrometer.
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    the fact that microorganism database in the apparatus includes only 13 Salmonella serotypes and according to the Kauffman-White classification there are more than 2,600 of Salmonella serotypes in the present time the results of MALDI identification related to serotype determination can differ from results of classical serotyping. Bacteria identification using direct protein method
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    confirmed that all tested microorganisms belong to the Salmonella genus (Table 1). Herewith, microorganism identification criterion was within 2,236–2,649, which is indicative of high probability of identification.
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    It was determined that 6 out of 27 studied Salmonella isolates demonstrated a typical peak with 100% intensity and m/z 4364 Dа in a protein spectrum. For all the rest isolates identification peak was at 6092 Dа. According to Zhou N. and Wang N.
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    a unique peak characterizing Salmonella paratyphi, is peak at m/z 6092 Da. In our test such peak was shown by all Salmonella isolates together with peak at 4363 Da. Performed mass-spectrometry of Enterobacteriaceae family reprsentatives demonstrated in Maldi Biotyper database (Table 2) determined that peak 4363+1 Da is typical of many representatives of Enterobacteriaceae family, whil
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    demonstrated in Maldi Biotyper database (Table 2) determined that peak 4363+1 Da is typical of many representatives of Enterobacteriaceae family, while peak at m/z 6092+1 Da is typical only of Salmonella и Trabulsiella guamensis bacteria. Trabulsiella bacteria were discovered in 1985 and originally were referred to Salmonella genus due to proximity of biochemical characteristics
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    . So, peak at m/z 6092 Da can be considered unique for Salmonella bacteria, and this information can be used when developing express-methods for microorganism detection immediately from material substance without preliminary isolation of pure cultures.
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    , peak at m/z 6092 Da can be considered unique for Salmonella bacteria, and this information can be used when developing express-methods for microorganism detection immediately from material substance without preliminary isolation of pure cultures. Besides, peaks used to characterize the microorganism’s family and genus, according to data presented by Dieckmann R. and Marlony B.
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    , peaks typical of 5 epidemically important Salmonella serotypes: Enteritidis, Typhimurium, Virchow, Infantis, Hadar, were determined. Furthermore, there were determined potential serovardetermining ions, which can be used as biomarkers for serotypes Choleraesuis, Heidelberg, Gallinarum (Table. 3).
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    Furthermore, there were determined potential serovardetermining ions, which can be used as biomarkers for serotypes Choleraesuis, Heidelberg, Gallinarum (Table. 3). Obtained results confirm R. Dieckmann and B. Malorny’s conclusion
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    as far as Choleraesuis (Salmonella Choleraesuis «Bashkiria», Salmonella Choleraesuis «Il», Salmonella Choleraesuis «Len», Salmonella Choleraesuis «Mordovia», Salmonella Choleraesuis «Vladimir») and Enteritidis (Salmonella Enteritidis «Ru 3», Salmonella Enteritidis «Gleb», Salmonella Enteritidis «Pel») serotypes are concerned (Table. 3).
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