The 10 references with contexts in paper A. Osipov S., O. Popova A., S. Larionova G., E. Timoshina Yu., А. Осипов С., О. Попова А., С. Ларионова Г., Е. Тимошина Ю. (2018) “Применение хроматографии гидрофильных взаимодействий для разделения гидроксикарбамида и мочевины // Hydrophylic interaction chromatography used for separation of hydroxycarbamide and urea” / spz:neicon:vedomostincesmp:y:2017:i:2:p:81-84

1
Monograph: Hydroxycarbamide Capsules. British Pharmacopoeia
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    Ãèäðîêñèêàðáàìèä (ÃÊ) îïèñàí â âåäóùèõ çàðóáåæíûõ ôàðìàêîïåÿõ. Äëÿ êîëè÷åñòâåííîãî îïðåäåëåíèÿ ÃÊ ïðèìåíÿþò õðîìàòîãðàôèðîâàíèå íà êîëîíêàõ Ñ18, â êà÷åñòâå ïîäâèæíîé ôàçû íàèáîëåå ÷àñòî èñïîëüçóþò âîäó
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    èëè ñìåñü ìåòàíîëà è âîäû (5:95) [2].  Ôàðìàêîïåå ÑØÀ [3, 4] äëÿ êîëè÷åñòâåííîãî îïðåäåëåíèÿ ÃÊ â ñóáñòàíöèè è ëåêàðñòâåííîé ôîðìå (êàïñóëû) èñïîëüçóþò èîí-ïàðíóþ õðîìàòîãðàôèþ íà êîëîíêàõ Ñ18 (ïîäâèæíàÿ ôàçà — ñìåñü áóôåðíîãî ðàñòâîðà ñ òåòðàáóòèëàììîíèÿ ãèäðîñóëüôàòîì (ðÍ 5,0) è ìåòàíîëà (85:15)).

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16. 2. Monograph: Hydroxycarbamide. European Pharmacopoeia. 8th ed.
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    Ãèäðîêñèêàðáàìèä (ÃÊ) îïèñàí â âåäóùèõ çàðóáåæíûõ ôàðìàêîïåÿõ. Äëÿ êîëè÷åñòâåííîãî îïðåäåëåíèÿ ÃÊ ïðèìåíÿþò õðîìàòîãðàôèðîâàíèå íà êîëîíêàõ Ñ18, â êà÷åñòâå ïîäâèæíîé ôàçû íàèáîëåå ÷àñòî èñïîëüçóþò âîäó [1] èëè ñìåñü ìåòàíîëà è âîäû (5:95)
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    .  Ôàðìàêîïåå ÑØÀ [3, 4] äëÿ êîëè÷åñòâåííîãî îïðåäåëåíèÿ ÃÊ â ñóáñòàíöèè è ëåêàðñòâåííîé ôîðìå (êàïñóëû) èñïîëüçóþò èîí-ïàðíóþ õðîìàòîãðàôèþ íà êîëîíêàõ Ñ18 (ïîäâèæíàÿ ôàçà — ñìåñü áóôåðíîãî ðàñòâîðà ñ òåòðàáóòèëàììîíèÿ ãèäðîñóëüôàòîì (ðÍ 5,0) è ìåòàíîëà (85:15)).

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Monograph: Hydroxycarbamide. The United States Pharmacopoeia. 39th ed.
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    Ãèäðîêñèêàðáàìèä (ÃÊ) îïèñàí â âåäóùèõ çàðóáåæíûõ ôàðìàêîïåÿõ. Äëÿ êîëè÷åñòâåííîãî îïðåäåëåíèÿ ÃÊ ïðèìåíÿþò õðîìàòîãðàôèðîâàíèå íà êîëîíêàõ Ñ18, â êà÷åñòâå ïîäâèæíîé ôàçû íàèáîëåå ÷àñòî èñïîëüçóþò âîäó [1] èëè ñìåñü ìåòàíîëà è âîäû (5:95) [2].  Ôàðìàêîïåå ÑØÀ
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    äëÿ êîëè÷åñòâåííîãî îïðåäåëåíèÿ ÃÊ â ñóáñòàíöèè è ëåêàðñòâåííîé ôîðìå (êàïñóëû) èñïîëüçóþò èîí-ïàðíóþ õðîìàòîãðàôèþ íà êîëîíêàõ Ñ18 (ïîäâèæíàÿ ôàçà — ñìåñü áóôåðíîãî ðàñòâîðà ñ òåòðàáóòèëàììîíèÿ ãèäðîñóëüôàòîì (ðÍ 5,0) è ìåòàíîëà (85:15)).

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Monograph: Hydroxyurea Capsules. The United States Pharmacopoeia. 39th ed.
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    Ãèäðîêñèêàðáàìèä (ÃÊ) îïèñàí â âåäóùèõ çàðóáåæíûõ ôàðìàêîïåÿõ. Äëÿ êîëè÷åñòâåííîãî îïðåäåëåíèÿ ÃÊ ïðèìåíÿþò õðîìàòîãðàôèðîâàíèå íà êîëîíêàõ Ñ18, â êà÷åñòâå ïîäâèæíîé ôàçû íàèáîëåå ÷àñòî èñïîëüçóþò âîäó [1] èëè ñìåñü ìåòàíîëà è âîäû (5:95) [2].  Ôàðìàêîïåå ÑØÀ
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    äëÿ êîëè÷åñòâåííîãî îïðåäåëåíèÿ ÃÊ â ñóáñòàíöèè è ëåêàðñòâåííîé ôîðìå (êàïñóëû) èñïîëüçóþò èîí-ïàðíóþ õðîìàòîãðàôèþ íà êîëîíêàõ Ñ18 (ïîäâèæíàÿ ôàçà — ñìåñü áóôåðíîãî ðàñòâîðà ñ òåòðàáóòèëàììîíèÿ ãèäðîñóëüôàòîì (ðÍ 5,0) è ìåòàíîëà (85:15)).

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    Ñëåäóåò îòìåòèòü, ÷òî äàííûé ìåòîä âåñüìà äëèòåëåí è òðóäîåìîê è íå ìîæåò áûòü ðåêîìåíäîâàí äëÿ ïîâñåäíåâíîãî, ðóòèííîãî ïðèìåíåíèÿ.  ñîîòâåòñòâóþùåé ìîíîãðàôèè Ôàðìàêîïåè ÑØÀ ñîäåðæàíèå ìî÷åâèíû â êàïñóëàõ ïðåïàðàòà íå ðåãëàìåíòèðîâàíî
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    . Ïðèìåíåíèå ìåòîäà ÂÝÆÕ äëÿ ðàçäåëåíèÿ ÃÊ è ìî÷åâèíû â çàðóáåæíûõ ôàðìàêîïåÿõ íå îïèñàíî. Íåîáõîäèìî îòìåòèòü, ÷òî â óñëîâèÿõ îáðàùåííî-ôàçîâîé õðîìàòîãðàôèè ÃÊ è ìî÷åâèíà íå ðàçäåëÿþòñÿ [5]. Ðàíåå áûëà ïîêàçàíà âîçìîæíîñòü ïðèìåíåíèÿ õðîìàòîãðàôè÷åñêèõ êîëîíîê ñ àìèíî-, äèîëüíûìè è íèòðèëüíûìè ñîðáåíòàìè äëÿ ðàçäåëåíèÿ ñìåñåé ÃÊ è ìî÷åâèíû [5, 6] â óñëîâèÿõ æèäêîñòíîé õðîìàòîãðàôèè ãèäðîôèëüí

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Osipov AS, Nechaeva EB, Pobedin OA. Hydrophilic interaction liquid chromatography used for the analysis of hydroxycarbamide. Drug Development and Registration 2015; (2): 140–4 (in Russian).
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     ñîîòâåòñòâóþùåé ìîíîãðàôèè Ôàðìàêîïåè ÑØÀ ñîäåðæàíèå ìî÷åâèíû â êàïñóëàõ ïðåïàðàòà íå ðåãëàìåíòèðîâàíî [4]. Ïðèìåíåíèå ìåòîäà ÂÝÆÕ äëÿ ðàçäåëåíèÿ ÃÊ è ìî÷åâèíû â çàðóáåæíûõ ôàðìàêîïåÿõ íå îïèñàíî. Íåîáõîäèìî îòìåòèòü, ÷òî â óñëîâèÿõ îáðàùåííî-ôàçîâîé õðîìàòîãðàôèè ÃÊ è ìî÷åâèíà íå ðàçäåëÿþòñÿ
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    . Ðàíåå áûëà ïîêàçàíà âîçìîæíîñòü ïðèìåíåíèÿ õðîìàòîãðàôè÷åñêèõ êîëîíîê ñ àìèíî-, äèîëüíûìè è íèòðèëüíûìè ñîðáåíòàìè äëÿ ðàçäåëåíèÿ ñìåñåé ÃÊ è ìî÷åâèíû [5, 6] â óñëîâèÿõ æèäêîñòíîé õðîìàòîãðàôèè ãèäðîôèëüíûõ âçàèìîäåéñòâèé (Hydrophilic Interaction Liquid Chromatography; HILIC).

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    Íåîáõîäèìî îòìåòèòü, ÷òî â óñëîâèÿõ îáðàùåííî-ôàçîâîé õðîìàòîãðàôèè ÃÊ è ìî÷åâèíà íå ðàçäåëÿþòñÿ [5]. Ðàíåå áûëà ïîêàçàíà âîçìîæíîñòü ïðèìåíåíèÿ õðîìàòîãðàôè÷åñêèõ êîëîíîê ñ àìèíî-, äèîëüíûìè è íèòðèëüíûìè ñîðáåíòàìè äëÿ ðàçäåëåíèÿ ñìåñåé ÃÊ è ìî÷åâèíû
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    â óñëîâèÿõ æèäêîñòíîé õðîìàòîãðàôèè ãèäðîôèëüíûõ âçàèìîäåéñòâèé (Hydrophilic Interaction Liquid Chromatography; HILIC). Ìî÷åâèíà è ÃÊ ÿâëÿþòñÿ àìèäàìè óãîëüíîé êèñëîòû, è èññëåäîâàíèå ðàçäåëåíèÿ äàííûõ ñîåäèíåíèé íà õðîìàòîãðàôè÷åñêîé êîëîíêå ñ àìèäíûìè ôóíêöèîíàëüíûìè ãðóïïàìè ìîæåò ïðåäñòàâëÿòü îïðåäåëåííûé òåîðåòè÷åñêèé èíòåðåñ.

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    Ñêîðîñòü ïîòîêà ýëþåíòà 1 ìë/ìèí èëè 0,8 ìë/ìèí (â çàâèñèìîñòè îò äèàìåòðà êîëîíîê). Ââîä îáðàçöîâ â îáúåìå 10 ìêë.  ðàáîòå èñïîëüçîâàëè äàííûå, ïîëó÷åííûå íà õðîìàòîãðàôè÷åñêèõ êîëîíêàõ Zorbax NH2 150 ́4,6 ìì, 5 ìêì
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    («Agilent Technologies», ÑØÀ) è Zorbax SB CN 150 ́4,6 ìì, 5 ìêì [6] («Agilent Technologies», ÑØÀ).  ðàáîòå ïðèìåíÿëè ñòàíäàðòíûå îáðàçöû ãèäðîêñèêàðáàìèäà è ìî÷åâèíû Åâðîïåéñêîé ôàðìàêîïåè. Àíàëèçèðîâàëè ïðåïàðàò ÃèäðîêñèêàðáàìèäËÝÍÑ, êàïñóëû 500 ìã (ÎÀÎ «Âåðîôàðì», Ðîññèÿ).

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     òàáëèöå 1 ïðèâåäåíû íåêîòîðûå ðåçóëüòàòû õðîìàòîãðàôèðîâàíèÿ ìîäåëüíîé ñìåñè ñòàíäàðòíûõ îáðàçöîâ ìî÷åâèíû è ÃÊ íà àìèäíîé êîëîíêå XBridge Amide è äèîëüíûõ êîëîíêàõ LiChrospher Diol è Inertsil Diol. Äëÿ ñðàâíåíèÿ ïðèâåäåíû ðåçóëüòàòû õðîìàòîãðàôèðîâàíèÿ ìî÷åâèíû è ÃÊ, ïîëó÷åííûå íà êîëîíêå Zorbax NH2
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    . Óâåëè÷åíèå âðåìåí óäåðæèâàíèÿ ìî÷åâèíû è ÃÊ, à òàêæå óëó÷øåíèå èõ ðàçäåëåíèÿ ñ âîçðàñòàíèåì äîëè àöåòîíèòðèëà â ïîäâèæíîé ôàçå óêàçûâàåò, ÷òî íà äàííûõ êîëîíêàõ èìååò ìåñòî íîðìàëüíî-ôàçîâûé ìåõàíèçì ðàçäåëåíèÿ â æèäêîñòíîé õðîìàòîãðàôèè ãèäðîôèëüíûõ âçàèìîäåéñòâèé (ðèñ. 1).

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Osipov AS, Nechaeva EB, Truhacheva LA. Chromatographic column with nitrile sorbent used in the analysis of hydroxycarbamide by hydrophilic interaction liquid chromatography. The Bulletin of the Scientific Centre for Expert Evaluation of Medicinal Products 2016; (3): 58–61 (in Russian).
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    Íåîáõîäèìî îòìåòèòü, ÷òî â óñëîâèÿõ îáðàùåííî-ôàçîâîé õðîìàòîãðàôèè ÃÊ è ìî÷åâèíà íå ðàçäåëÿþòñÿ [5]. Ðàíåå áûëà ïîêàçàíà âîçìîæíîñòü ïðèìåíåíèÿ õðîìàòîãðàôè÷åñêèõ êîëîíîê ñ àìèíî-, äèîëüíûìè è íèòðèëüíûìè ñîðáåíòàìè äëÿ ðàçäåëåíèÿ ñìåñåé ÃÊ è ìî÷åâèíû
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    â óñëîâèÿõ æèäêîñòíîé õðîìàòîãðàôèè ãèäðîôèëüíûõ âçàèìîäåéñòâèé (Hydrophilic Interaction Liquid Chromatography; HILIC). Ìî÷åâèíà è ÃÊ ÿâëÿþòñÿ àìèäàìè óãîëüíîé êèñëîòû, è èññëåäîâàíèå ðàçäåëåíèÿ äàííûõ ñîåäèíåíèé íà õðîìàòîãðàôè÷åñêîé êîëîíêå ñ àìèäíûìè ôóíêöèîíàëüíûìè ãðóïïàìè ìîæåò ïðåäñòàâëÿòü îïðåäåëåííûé òåîðåòè÷åñêèé èíòåðåñ.

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    Ââîä îáðàçöîâ â îáúåìå 10 ìêë.  ðàáîòå èñïîëüçîâàëè äàííûå, ïîëó÷åííûå íà õðîìàòîãðàôè÷åñêèõ êîëîíêàõ Zorbax NH2 150 ́4,6 ìì, 5 ìêì [5] («Agilent Technologies», ÑØÀ) è Zorbax SB CN 150 ́4,6 ìì, 5 ìêì
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    («Agilent Technologies», ÑØÀ).  ðàáîòå ïðèìåíÿëè ñòàíäàðòíûå îáðàçöû ãèäðîêñèêàðáàìèäà è ìî÷åâèíû Åâðîïåéñêîé ôàðìàêîïåè. Àíàëèçèðîâàëè ïðåïàðàò ÃèäðîêñèêàðáàìèäËÝÍÑ, êàïñóëû 500 ìã (ÎÀÎ «Âåðîôàðì», Ðîññèÿ).

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    Ïðèìåíåíèå õðîìàòîãðàôèè ãèäðîôèëüíûõ âçàèìîäåéñòâèé äëÿ ðàçäåëåíèÿ ãèäðîêñèêàðáàìèäà è ìî÷åâèíû Ðèñ. 2.Õðîìàòîãðàììà ïðåïàðàòà «Ãèäðîêñèêàðáàìèä-ËÝÍÑ», êàïñóëû 500 ìã ñ äîáàâêîé ìî÷åâèíû. Óñëîâèÿ àíàëèçà: êîëîíêà Zorbax SB CN 150 ́4,6 ìì (5 ìêì); ïîäâèæíàÿ ôàçà — àöåòîíèòðèë–âîäà (93:7); ñêîðîñò üïîòîêà — 1 ìë/ìèí; äåòåêòèðîâàíèå 195 íì
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    Ðèñ. 3.Õðîìàòîãðàììà ïðåïàðàòà «Ãèäðîêñèêàðáàìèä-ËÝÍÑ», êàïñóëû 500 ìã ñ äîáàâêîé ìî÷åâèíû. Óñëîâèÿ àíàëèçà: êîëîíêà XBridge Amide 150 ́4,6 ìì (3,5 ìêì); ïîäâèæíàÿ ôàçà — àöåòîíèòðèë–âîäà (93:7); ñêîðîñò üïîòîêà — 1 ìë/ìèí; äåòåêòèðîâàíèå 195 íì ÎÁ ÀÂÒÎÐÀÕ Ôåäåðàëüíîå ãîñóäàðñòâåííîå áþäæåòíîå ó÷ðåæäåíèå «Íàó÷íûé öåíòð ýêñïåðòèçû ñðåäñòâ ìåäèöèíñêîãî ïðèìåíåíèÿ» Ìèíèñòåðñòâà çäðàâîîõðàíåíèÿ Ðîññè

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Osipov AS, Nechaeva EB, Pobedin OA. Application of a chromatographic column with a diol sorbent for the analysis of platinum coordination compounds. Pharmaceutical Chemistry Journal 2013; 47(6): 51–3 (in Russian).
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    ÐÅÇÓËÜÒÀÒÛ È ÎÁÑÓÆÄÅÍÈÅ Â óñëîâèÿõ æèäêîñòíîé õðîìàòîãðàôèè ãèäðîôèëüíûõ âçàèìîäåéñòâèé ñâîéñòâà êîëîíîê ñ àìèíîè äèîëüíûìè ñîðáåíòàìè ìîãóò áûòü, â çàâèñèìîñòè îò àíàëèçèðóåìûõ îáúåêòîâ, êàê áëèçêè
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    , òàê è êàðäèíàëüíî îòëè÷àòüñÿ îò êîëîíîê ñ íèòðèëüíûìè ñîðáåíòàìè [9, 10].  òàáëèöå 1 ïðèâåäåíû íåêîòîðûå ðåçóëüòàòû õðîìàòîãðàôèðîâàíèÿ ìîäåëüíîé ñìåñè ñòàíäàðòíûõ îáðàçöîâ ìî÷åâèíû è ÃÊ íà àìèäíîé êîëîíêå XBridge Amide è äèîëüíûõ êîëîíêàõ LiChrospher Diol è Inertsil Diol.

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Osipov AS, Nechaeva EB. Application of chromatographic columns with nitrile and phenyl sorbents to the analysis of platinum coordination compounds. Pharmaceutical Chemistry Journal 2014; 48(8): 45–8 (in Russian).
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    ÐÅÇÓËÜÒÀÒÛ È ÎÁÑÓÆÄÅÍÈÅ Â óñëîâèÿõ æèäêîñòíîé õðîìàòîãðàôèè ãèäðîôèëüíûõ âçàèìîäåéñòâèé ñâîéñòâà êîëîíîê ñ àìèíîè äèîëüíûìè ñîðáåíòàìè ìîãóò áûòü, â çàâèñèìîñòè îò àíàëèçèðóåìûõ îáúåêòîâ, êàê áëèçêè
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    , òàê è êàðäèíàëüíî îòëè÷àòüñÿ îò êîëîíîê ñ íèòðèëüíûìè ñîðáåíòàìè [9, 10].  òàáëèöå 1 ïðèâåäåíû íåêîòîðûå ðåçóëüòàòû õðîìàòîãðàôèðîâàíèÿ ìîäåëüíîé ñìåñè ñòàíäàðòíûõ îáðàçöîâ ìî÷åâèíû è ÃÊ íà àìèäíîé êîëîíêå XBridge Amide è äèîëüíûõ êîëîíêàõ LiChrospher Diol è Inertsil Diol.

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Osipov AS, Nechaeva EB, Mironova MM, Kovaleva EL. Use of hydrophilic interaction liquid chromatography to separate butylhydroxyanisole isomers. Pharmaceutical Chemistry Journal 2015; 49(3): 50–2 (in Russian).
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    ÐÅÇÓËÜÒÀÒÛ È ÎÁÑÓÆÄÅÍÈÅ Â óñëîâèÿõ æèäêîñòíîé õðîìàòîãðàôèè ãèäðîôèëüíûõ âçàèìîäåéñòâèé ñâîéñòâà êîëîíîê ñ àìèíîè äèîëüíûìè ñîðáåíòàìè ìîãóò áûòü, â çàâèñèìîñòè îò àíàëèçèðóåìûõ îáúåêòîâ, êàê áëèçêè [7, 8], òàê è êàðäèíàëüíî îòëè÷àòüñÿ îò êîëîíîê ñ íèòðèëüíûìè ñîðáåíòàìè
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    .  òàáëèöå 1 ïðèâåäåíû íåêîòîðûå ðåçóëüòàòû õðîìàòîãðàôèðîâàíèÿ ìîäåëüíîé ñìåñè ñòàíäàðòíûõ îáðàçöîâ ìî÷åâèíû è ÃÊ íà àìèäíîé êîëîíêå XBridge Amide è äèîëüíûõ êîëîíêàõ LiChrospher Diol è Inertsil Diol.

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Osipov AS, Nechaeva EB, Truhacheva LA. Hydrophilic interaction liquid chromatography used for the analysis of organic nitrates. Drug Development and Registration 2016; (3): 108–11 (in Russian). AUTHORS Federal State Budgetary Institution «Scientific Centre for Expert Evaluation of Medicinal Products» of the Ministry of Health
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  1. In-text reference with the coordinate start=5881
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    ÐÅÇÓËÜÒÀÒÛ È ÎÁÑÓÆÄÅÍÈÅ Â óñëîâèÿõ æèäêîñòíîé õðîìàòîãðàôèè ãèäðîôèëüíûõ âçàèìîäåéñòâèé ñâîéñòâà êîëîíîê ñ àìèíîè äèîëüíûìè ñîðáåíòàìè ìîãóò áûòü, â çàâèñèìîñòè îò àíàëèçèðóåìûõ îáúåêòîâ, êàê áëèçêè [7, 8], òàê è êàðäèíàëüíî îòëè÷àòüñÿ îò êîëîíîê ñ íèòðèëüíûìè ñîðáåíòàìè
    Exact
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    .  òàáëèöå 1 ïðèâåäåíû íåêîòîðûå ðåçóëüòàòû õðîìàòîãðàôèðîâàíèÿ ìîäåëüíîé ñìåñè ñòàíäàðòíûõ îáðàçöîâ ìî÷åâèíû è ÃÊ íà àìèäíîé êîëîíêå XBridge Amide è äèîëüíûõ êîëîíêàõ LiChrospher Diol è Inertsil Diol.